Quantification by UV spectrophotometer
A simple linear regression curve was plotted using standard capsaicin, purchased from Sigma Chemical. A stock solution of 1mg/ml capsaicin in ethanol prepared and different concentrations from 10 µg to 100 µg were prepared from the stock solution. The optical density was recorded at 280 nm. The linear regression equation was generated using the online Statistics and forecasting software (www. wessa.net). The capsaicinoid extracted from solvents was estimated by UV visible spectrophotometer (Hitachi- U1800). The crude extract was diluted to 300X using the respective solvent. The optical density was recorded at 280 nm. The capsaicinoid concentrations in the samples were calculated using capsaicin linear regression equation and it was expressed as µg/ml of capsaicin and finally converted to Scoville
Heat Unit.