2.5.Analytical Methods
The samples were centrifuged at 10,000g for 10 min. The supernatants were analyzed for total acidity [8],
lactic acid concentration [9] and starch concentration [10]. For biomass concentration determination, the
harvested cells were washed with the distilled water and dried at 80°C until of a constant weight. Then, the
dried cells were weighed. All SSF experiments were performed in triplicate.