In summary, we investigated the effects of feed
restriction and different energy and protein contents of
the diet on ghrelin gene expression in broiler chicken.
We have characterized chicken ghrelin cDNA in
proventriculus tissue in broiler chicken. We also found
that ghrelin gene expression is differently suppressed
by diet manipulations. Additional studies are necessary
to investigate the role of nutrition on ghrelin gene
expression in proventriculus tissue in broiler chicken.