No feed or water was allowed during each sampling period.
Horses had not received furosemide for at least 3
days before the treatment/collection periods. Blood
samples thus collected were centrifuged in a swinging
bucket-type centrifuge at a speed of 1200 relative centrifugal
force for 10 minutes and refrigerated at 4°C
until tCO2 analysis within 24 hours after collection.