The
expression of TrCel3B resulted in a low level of b-glucosidase
activity, both in the fraction containing the precipitated cells and
in the culture supernatant (Fig. 3B). The activity level of TrCel3B
was higher compared to TrCel3A, but was significantly lower than
AaBGL1, which showed robust pNPGlc activity, especially in the
cell pellet fraction, which was suspended in acetate buffer (pH
5.0) with the same volume of culture medium. We decided to
characterize TrCel3B and AaBGL1 and evaluate their suitability for
the production of bioethanol.