One of the primary aims was to provide initial isolation data, comparing the non-actinomycete, the actinomycete and the fungal populations that were isolated from both the paunch and colon regions of the hindgut of M. viator, on four different media, each at four different pHs. Another aim was to provide a detailed comparison of both the “Original” and “Duplicate” isolates , in terms of the preference of the actinomycete isolates for different isolation media and pHs, and to determine the distribution of the isolates obtained from the paunch and colon.