For cell cycle synchronization 200 ml of thymidine solution (8 mg/ml in PBS) was added at 56 h of culture, and colcemide (0.10 mg/ml) was added 4.5 h before harvest to arrest lymphocytes at metaphase.
For cellcycle synchronization 200 ml of thymidine solution(8 mg/ml in PBS) was added at 56 h of culture, andcolcemide (0.10 mg/ml) was added 4.5 h before harvestto arrest lymphocytes at metaphase.