All participants in the exercise groups underwenta 12-wk supervised aerobicexercise program three times per week, lasting 45 min each time according topre-established heart rate (65%–75% of their HR max). Each session was super-vised by one researcher in a municipal sport center. The participants wore aportable heart rate monitor (Polar RS400, Finland) and heart rate was recordedevery 5 min to check exercise intensity. Each session consisted of 5-minwarm-upand stretching, followedby45 min of aerobicrunning and finally5 min at the endfor stretching.Groups 1 and 3 were given precise instructions on how to follow a diet thatmaintained their caloric and nutrient intakes but significantly reduced AGEcontent; the latter was achieved mostly by changing cooking methods in foodpreparation to avoid exposure to dry heat such as frying, broiling, grilling, androasting and to favor cooking with lower temperatures and high-water, contentas in stewing and poaching, as previously described [22]. The second groupcontinued consuming their habitual meals. The energy and nutrient consump-tion was calculated with the program Nutrikcal (University of Monterrey,M ? exico) and AGE intake was calculated from a database of w560 foods that listedAGE values and expressed as AGE kU/d [23].Of the initial 75 participants, only 43 completed the intervention (15 in thediet plus exercise group, 14 in the exercise group, and 14 in the diet group). Theremainder of the participants were excluded because of lack of adherence withthe diet or exercise (attendance to <80% of the exercise sessions), or both. Thesample size was calculated according to expected changes in serum AGEs, aspreviously reported [24] and considering a ¼ 0.05 and b ¼ 0.20 and power of 80%.
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