RPMI Medium 1640 (RPMI 1640) Medium powder with L-glutamine, Fetal Bovine Serum (FBS), Penicillin-Streptomycin (P/S), trypsin-EDTA and trypan blue were purchased from Gibco, USA. Phosphate Buffer Saline (PBS) was from Amresco, USA.
RPMI Medium 1640 (RPMI1640) Medium powder with L-glutamine, FetalBovine Serum (FBS), Penicillin-Streptomycin(P/S), trypsin-EDTA and trypan blue werepurchased from Gibco, USA. Phosphate BufferSaline (PBS) was from Amresco, USA.