, YIpdAGSGd was linear ized by digesting the d sequences with XhoI (Fig. 1),
after which the resultant fragments (9.5 kb) harboring
the ADC1p-GA1 and ADC1p-GAM1 gene cassettes
flanked by the d sequences could be integrated into d
sequences dispersed throughout the S. cerevisiae
genome to generate multicopy integrants