3.1. Extraction and fractionation
An yield of about 6.452 g (25.808%) was obtained from the
methanolic extract of C. trinodis. The combined methanolic
extract was further fractionated with DCM, ethyl acetate
and butanol, which yielded 0.4140 g of DCM fraction,
0.202 g of ethyl acetate fraction and 0.151 g of butanol-soluble
fraction (Table 1). The DCM fraction with the higher antioxidant
potential was further fractionated in a silica gel column
chromatography and 45 fractions were collected. All the 45
fractions were subjected to TLC, leading to the production
of 7 subfractions namely F1–F7.