Stages of samples content analysis
Firstly, the ecstasy tablets were weighed using a digital
scale. Then, the tablets were powdered using a porcelain
mortar. Considering the difference in the tablets weights, 40
g of each tablet was put into appropriate falcon tubes.
Subsequently, 50 mL monopotassium phosphate buffer
solution containing 1% triethylamine with adjusted pH using phosphoric acid, 3/2 (1 M) was added. In order to
effectively mix the materials and extract the MDMA, the
mixture was put in ultrasonic device for 30 minutes. Then,
the falcon tubes were centrifuged at 2000g for five minutes.
Ultimately, the samples were filtered using syringe filters
and were injected into the HPLC column. Each sample was
analyzed in triplicate (Figure 2).