Fig. 5. Effects of cold stress conditions on HSP70C, HSP70A and HSP90A transcript accumulation. (A) Quantification of the HSP70C and HSP70A transcript levels by real-timePCR. Relative expression levels were normalized with the gene expression of RACK1. Accumulation of the HSP70C and HSP70A transcripts during heat shock at 40◦C isgiven for a comparison (insert). (B) Quantification of the HSP90A transcript levels by real-time PCR. Relative expression levels were normalized with the gene expression ofRACK1. Accumulation of the HSP90A transcripts during heat shock at 40◦C is given for a comparison (insert). Relative expression levels were normalized as in (A). Significantdifferences at the 95% level with respect to the control are indicated with **. Cells were treated as described in the legend to Fig. 2A.