controlled room. Likewise, cyanide degradation activity
of anaerobic pond sediment was carried out also in the
serum bottles. A known amount of sludge obtained from Pond #4 sediment layer was kept in serum bottles
and filled with 70 ml of wastewater having substrate and
nutrient similar to those mentioned for the SMA test. A
stock cyanide solution was added into each serum bottle
to achieve cyanide concentrations of 10 and 20 mg/l
respectively. The bottle was then purged with N2 gas
and immediately sealed with a rubber septum and an
aluminum cap. The bottle was kept in a 30 C temperature-
controlled room. Samples were taken with
Hamilton syringe at every 8 h interval for 48 h and
analyzed for cyanide content.