Acute inflammation was produced by subplantar injection of 0.1 mL of 0.1% histamine into the right
hind paw of each rat in the control and treatment groups. The degree of edema was measured before injection
and at the time points of 30, 60, 120, 180, 240 and 300 min after injection. Changes of peritoneal vascular
permeability were studied using Evans blue dye as a detector. Vascular permeability was evaluated by the amount
of dye leakage into the peritoneal cavity in rats. To evaluate the inhibitory effect of AEBO on biochemical mediators
of vascular permeability, the levels of nitric oxide (NO) and vascular endothelial growth factor (VEGF) were
determined in histamine-treated paw tissues. The major constituents of AEBO were determined by gas
chromatography–mass spectrometry (GC-MS) analysis.