Root rot lesions were observed on the basal stem. The root of each soybean plant was collected, washed under running tap water, and assessed for the presence and severity of root rot symptoms on a 0–4 scale according to Zhou et al. (2014), where: 0 = no symptoms, 1 = mild symptoms (discoloration but no visible lesions), 2 = obvious lesions, 3 = severe lesions on the stem and diminished plant vigor, and 4 = stem rotten, plant dead. The soybean plants of 1–4 scale are pathogenetic plant. Disease Incidence Statistics were dealed with 100 plants of each soil sample.