2.3. Blood collection
Three milliliters (3 ml) of venous blood were collected within
the first 24 h post ICU admission. Blood samples were either collected
in red topped Vacutainer tubes (BD, Mississauga, Ontario,
Canada) in order to collect serum, or in Vacutainer tubes containing
either 0.129 M (3.8%) trisodium citrate or EDTA for the collection
of plasma. Whole blood was allowed to clot by leaving it
undisturbed at room temperature for 15–30 min. Serum was collected
after centrifugation at 1000g for 10 min at 4 C, apportioned
into 0.5 ml aliquots, and stored at 80 C until used. Alternatively,
the citrate or EDTA-containing tubes were centrifuged for 10 min
at 1000g at 4 C to remove cells from plasma. Subsequently, plasma
was collected, apportioned into 0.5 ml aliquots and stored at
80 C until used.
2.3. Blood collection
Three milliliters (3 ml) of venous blood were collected within
the first 24 h post ICU admission. Blood samples were either collected
in red topped Vacutainer tubes (BD, Mississauga, Ontario,
Canada) in order to collect serum, or in Vacutainer tubes containing
either 0.129 M (3.8%) trisodium citrate or EDTA for the collection
of plasma. Whole blood was allowed to clot by leaving it
undisturbed at room temperature for 15–30 min. Serum was collected
after centrifugation at 1000g for 10 min at 4 C, apportioned
into 0.5 ml aliquots, and stored at 80 C until used. Alternatively,
the citrate or EDTA-containing tubes were centrifuged for 10 min
at 1000g at 4 C to remove cells from plasma. Subsequently, plasma
was collected, apportioned into 0.5 ml aliquots and stored at
80 C until used.
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