Indirect Immunofluorescence
Paraffin-embedded sections of the fetal brain
tissue and brain tissue of an autopsied man as a
negative control were incubated with serum obtained
from the mother of the fetus (dilution,
1:10), followed by antihuman IgG antibodies
labeled with fluorescein isothiocyanate (FITC)
(dilution, 1:50). In addition, fetal brain tissue
was incubated with a serum obtained from a
healthy blood donor, as well as with FITC-labeled
antihuman IgG antibodies only.