2. Methods
2.1. Inoculum preparation
R. oryzae (CCT 1217) was obtained from the Foundation André
Tosello (FAT), Campinas, Brazil. The cultures were maintained at
4 C on potato dextrose agar (PDA, Acumedia). Spores from 7-
day-old cultures grown at 30 C were by suspending them in
0.2 mL (v/v) of Tween 80. The concentration of spores in the suspension
was estimated by enumeration in a Neubauer chamber.