Since the starch content was almost unvaried before and after
thermal treatment, when determined by chemical hydrolysis, the
general decreasing trend found by enzymatic analysis was
considered ascribable to thermal retrogradation (Acquistucci et al.,
2009). Unfortunately, the presence of retrograded starch strongly
affected the analytical performances of the enzymatic assay used
for amylose determination, an issue not reported by the instructions
and not known when the assay was applied. Under this
condition, the absolute amount of amylose in dried samples cannot
be given, but the percentage decrease after thermal treatment can
be easily extrapolated as the ratio between amylose absorbance
values read for each sample after and before the treatment.