Biomedical experiment often requires the use of live or recently deceases tissue samples. However, these tissue samples do not always get used in the experimental process, and thus go to waste. A cost effective, efficient means of best preserving skeletal muscle tissue for biophysical research is the goal of the research. Cryopreservation, or significantly dropping the temperature of a sample to essentially stop all cell function, is believed to be the best means of storing specimens. Freezing tissue sample exists as an intricate and delicate process in order for samples to maintain structural integrity. A major barrier is the formation of ice within cells. Intracellular ice ill expand when frozen, tearing the cellular structure apart. Therefore, rates of freezing, level of cryopreservants and tying muscles to capillary tubes were studied. Working in Dr. Kenneth Campbell’s laboratory in the Department of Physiology with Senior Lab Technician Ben Lawson, a cryopreserving solution which appears to maintain the structure of the tissue sample was search for. Also, finding a means of insulating the specimen vials to control freezing rate was performed. The sample were determined effectively stored if mechanical assays of stored tissue had on significant difference in physical properties than recently excised tissue. Results suggest that a slow freezing rate with a high rate of thawing in high concentrations of cryopreservants and being tied to capillary tubes allows for the best structurally sound samples. Finding a method of preserving tissue samples allows decreases the amount of waste due to degraded muscle tissue