3. Results and discussion
3.1. Mobile phase selection
Five mobile phases consisting of acetonitrile:methanol:water (8:27:65, 17:0:83, 17:13:70, 8:20:72 and 10:20:70) were tried.Fig. 1 shows the effect of mobile phase compositions on the retention
times of the aflatoxins. Aflatoxin G1 standard cannot be detected by the second and the fourth mobile phases. Shorter