Evaluation of total antioxidant activity by Phosphomolybdenum method
The total antioxidant capacity of the fungal extracts was evaluated according to the method
described by Prieto et al. 1999. An aliquot of 0.5mL of samples solution was combined with 4.5mL
of reagent solution (0.6M sulfuric acid, 28mM sodium phosphate, and 4mM ammonium
molybdate). Methanol (0.3mL) in the place of extract is used as the blank. The tubes were
incubated in a boiling water bath at 95°C for 90 min. After the samples were cooled to room
temperature, the absorbance of the aqueous solution of each sample was measured at 695nm
against blank in ELICO SL 159 UV-VIS Spectrophotometer. The total antioxidant activity is
expressed as the number of gram equivalents of ascorbic acid. The higher absorbance value
indicated higher antioxidant activity.