2.4. Microbiological analysis
Each sample (25 g) was put in a sterile stomacher bag, homo- genised with sterile peptone water (0.1%, 225 ml) and serial di-lutions of samples were prepared. Each sample (0.1 ml) was used for spread plating. Aerobic mesophilic count was enumerated from standard plate count agar (PCA), which was incubated at 37?C for 2 days. Aerobic psychotropic count was enumerated from PCA with10 days incubation at 7?C. Yeasts and moulds were counted basedon potato dextrose agar (PDA) with incubation at 25?C for 4 days.The results were expressed as log CFU/g sample