Calibration
An IS (methyl nonadecanoate, 0.05 mg/ml) method was used for
the calibration curves construction. Table 1 reports the six concentrations
of FAMEs used for calibration curves, the retention times
for the individual FAME, and correlation coefficients of the calibration
plots. According to the Eurachem Guide (1998) each point of
calibration curve was obtained by six replicates.