By immunoblotting, Bax protein in hippocampus is increased transiently at 6 h postischemia but then returns to control levels (154). We have not found changes in Bax protein levels in hippocampus at 1 and 7 days after ischemia, but we have observed an increase in Bax in cerebellum at 7 days after ischemia (149). It is possible that this change in Bax reflects apoptosis of granule neurons (rather than Purkinje neurons) in cerebellum (149). However, a sustained postischemic increase in Bax protein levels has been shown in hippocampus but not cerebellum (160), although the contributions of different cells to this observation have not been identified.