The nitrogen, vitamin, and carbon sources are provided by Enzymatic Digest of Animal Tissue and Beef Heart. The low concentration of Dextrose is sufficient as the energy source, but not high enough to accumulate toxic metabolites. Sodium Chloride maintains the osmotic balance. Solid meat particles provide favorable growth conditions for anaerobes due to reducing action of -SH (sulfhydryl) groups of muscle protein.2-4 Sulfhydryl groups are more accessible in denatured proteins, therefore use of cooked meat particles is preferred.8