Microwave alkali pretreated sugarcane bagasse was used as a substrate for production of cellulolytic
enzymes, needed for biomass hydrolysis. The pretreated sugarcane bagasse was enzymatic hydrolyzed by
crude unprocessed enzymes cellulase (Filter paper activity 9.4 FPU/g), endoglucanase (carboxymethylcellulase,
148 IU/g), b-glucosidase (116 IU/g) and xylanase (201 IU/g) produced by Aspergillus
flavus using pretreated sugarcane bagasse as substrate under solid state fermentation. Concentrated
enzymatic hydrolyzate was used for ethanol production using Saccharomyces cerevisiae immobilized on
various matrices. The yield of ethanol was 0.44 gp/gs in case of yeast immobilized sugarcane bagasse,
0.38 gp/gs using Ca-alginate and 0.33 gp/gs using agar-agar as immobilization matrices. The immobilized
yeast studied up to 10 cycles in case of immobilized sugarcane bagasse and up to 4 cycles in case of agaragar
and calcium alginate for ethanol production under repeated batch fermentation study.