According to the study of Emine Mine Soylu in the topic “ VIn vitro and in vivo antifungal activities of the essential oils of various plants against tomato grey mould disease agent Botrytis cinerea”
Based on the study The effects of different concentrations of volatile (A) and contact (B) phase of essential oil
The controls received the same quantity of ethanol and Tween 20 mixed with PDA. B. cinerea was inoculated immediately by plating in the centre of each plate. The Petri dishes were incubated in the dark at 22 °C.
Add the oil in the treated petri plates at different concentrations using three types of essential oils such as origanum , lavender and rosemary . Is divided into 2 ways such as volatile and contact
by volatile to use different concentrations of essential oils were added to sterile filter papers and placed on the inner surface of the lid of Petri dishes to obtain final concentrations of 0.05 to 1.6 μm/ml air. The Petri dishes were sealed immediately with parafilm to prevent loss of essential
and result in this …
by contact to use the essential oils were dispersed as an emulsion in water using ethanol and Tween 20 and added to PDA immediately before it was emptied into the glass Petri dishes at a temperature of 40–45 °C. The concentrations tested were 0.4 to 25.6 μg/ml.
and result in this …