Seed material (0.5 g) was homogenized in 5 ml of 0.1%
trichloroacetic acid (TCA) and centrifuged at 20,000 g for
10 min. To 1 ml of the extract, 4 ml of 0.5% (w/v) thiobarbituric
acid (TBA) in 20% (w/v) TCAwas added. The homogenate was
incubated at 95 8C for 30 min, cooled on ice and centrifuged at
16,000 g for 30 min and MDA content (mmol g/FW) was
spectrometrically determined at 452 nm according to Heath and
Parker (1968).