In the present study, optimal acid hydrolysis condition concerning
isoflavone content from soybean seeds was the first evaluated
using drying oven and microwave assisted methods. We showed a
fast and reliable acid hydrolysis method by microwave to estimate
isoflavone content in soybean through three parameters, acid
hydrolysis methods, hydrolysis time, and isoflavone extraction
time. It has also been confirmed that the optimum condition was
hydrolysed by 1 N HCl for 50 min at 100 C using microwave assisted
method. This study suggests that the microwave assisted
method is an attractive candidate for use in converting isoflavone
glycosides to their aglycones. Additionally, it can be applicable in
the process of isoflavone deglycosylation from soybeans and soy
food products.