The main purpose of the drug therapy of any dis- ease is to maintain the desired therapeutic concentra- tion of the drug for the entire duration of the treat- ment. Essential oils are secondary metabolites of plants with very complex structures based on the terpenoid substances, which show great antibacterial activity, especially from the plants belonging to Lamiaceae family, documented by number of studies [1,2]. Thyme essential oil shows very strong antimicrobial effect on large number of bacteria and fungi and it is very inter- esting in antimicrobial therapy in the era of antimic- robial resistance [3]. Essential oils, thyme oil as well, are volatile compounds which easily evaporate, and decompose during drug and cosmetics formulation, and direct exposure to heat, pressure, oxygen or light [4]. Their encapsulation inside the particles is very important to protect bioactive components that are consisted in essential oils, from the direct contact of the external factors that can change their biochemical properties. Encapsulation is, thus, the best way to retain functional properties of the essential oil and to reach promising therapeutic effect, that essential oils show in in vitro testing