Sodium thiosulphate (150 lL)
was used to neutralise the excess of bromine. After adding NaCl
(4 g), a double liquid–liquid extraction with ethyl acetate/n-hexane
4:1 (v/v) (10 + 5 mL) was performed. The collected organic extract
was dried with anhydrous Na2SO4, centrifuged, concentrated
(N2, 60 C) to about 0.5 mL and injected in the gas chromatograph