Several PCR protocols have been developed to characterize the different vancomycin resistance genes harbored by enterococcal strains.
In this study, we designed a MPCR protocol for the
simultaneous identification of Enterococcus isolates at the
genus level and detection of vancomycin resistance genes.
Moreover, the protocol was combined with a fast DNA
extraction procedure, which permitted rapid isolation of
DNA by boiling from a few colonies.