The specific primers of Pp14-3-3 were designed with the software Primer Premier 5, and the sequence of
forward and reverse primer was 5'-CGAGACAATCTGACACTCTGGACTTC-3' and 5'-
ACCCACATGCCATCTATTACAAAGG-3', respectively. A tobacco actin gene (AB158612.1) was included
in Real time PCR as the internal control. The detailed protocol of Real time PCR was described