Chitosan is known as a cheap, eco-friendly and efficient biosorbent for pollutant mixtures. In the treatment of textile effluents treatment, it has been widely applied for dye removal and/or
decolorization [1]. The amino (–NH2) and hydroxyl (–OH) groups of chitosan serve as the active sites for biosorption. The chemical modification of this water-insoluble material can be done easily because of the presence of these groups [2–4]. Chitosan is soluble in an acetic acid solution, and its hydrogel beads are precipitated and formed in NaOH solution. The elemental structure of chitosan has no difference after this acidic modification. The chitosan beads
(CBs) have a higher adsorption capacity than the raw chitosan flakes.Laccase has been of interest in recent years for synthesizing various dyes and decolorizing industrial textile effluents [5]. It catalyzes the oxidation of substrates coupled with the reduction of
molecular oxygen from water. Laccase was effectively and popularly used for dye removal [6,7]. Under alkaline conditions, the hydroxide ion causes enzyme inhibition [8].