The microbiological analyses revealed the presence of various pathogenic microorganisms in manure in addition to the common microflora of animal intestines. Persistence of pathogens in manures and in the water environment is one of the most important factors for infection transmission. It was found that zoonotic pathogens can survive in such environments up to 4 months, depending on the type of manure, temperature, pH,oxygen level, ammonia concentration and the presence of competing organisms (Jones, 1976; Guan and Holley, 2003).