Preparation ofmaterial
In thisnewstrategy,N-acetylmuramideglycanohydrolaseisintroducedasthecriticalenzyme
which willreactwiththepolymericmatrixofthebiofilm,reduceitsadherenceandmakethebiofilm
detach fromthesurface.Inthisstudy,theoptimalpHandtemperatureforreactionis101 and
4528C, respectively.Thetemperatureof4528C isusedthroughoutthewholeapplicationprocedure.
The materialwasprocessedinthefollowingmanner