Antigene Action. Next we investigated the gene silencing
function of the PNA
NFκB
delivered into the cells by MPOCs.
We incubated RAW 264.7 cells with each tested sample
(PNA
random
and PNA
either alone or with MPOCs),
stimulated the cells with lipopolysaccharide (LPS) plus murine
recombinant interferon gamma (rmIFN-γ), then monitored the
amount of Il6 mRNA by the quantitative reverse transcription
polymerase chain reaction (qRT-PCR) (Figure 6a) and amount
of IL-6 protein by enzyme-linked immunosorbent assay
(ELISA) (Figure 6b). The result showed that without
MPOC, the presence of the two PNAs did not affect Il6
mRNA level. Under the unstimulated condition, MPOCs (5.0
mg/L) caused a small increase in the level of Il6 mRNA. It was
possible that at the concentration used, MPOCs could mildly
stimulate Il6 mRNA expression. Elucidation on this point is,
however, beyond the scope of this report. Comparing between
the cells treated with PNA
NFκB
/MPOCs and those treated
with PNA
NFκB
random
/MPOCs, the latter showed 70% less increase of
Il6 mRNA level upon LPS/rmIFN-γ stimulation (Figure 6a).
Antigene Action. Next we investigated the gene silencingfunction of the PNANFκBdelivered into the cells by MPOCs.We incubated RAW 264.7 cells with each tested sample(PNArandomand PNAeither alone or with MPOCs),stimulated the cells with lipopolysaccharide (LPS) plus murinerecombinant interferon gamma (rmIFN-γ), then monitored theamount of Il6 mRNA by the quantitative reverse transcriptionpolymerase chain reaction (qRT-PCR) (Figure 6a) and amountof IL-6 protein by enzyme-linked immunosorbent assay(ELISA) (Figure 6b). The result showed that withoutMPOC, the presence of the two PNAs did not affect Il6mRNA level. Under the unstimulated condition, MPOCs (5.0mg/L) caused a small increase in the level of Il6 mRNA. It waspossible that at the concentration used, MPOCs could mildlystimulate Il6 mRNA expression. Elucidation on this point is,however, beyond the scope of this report. Comparing betweenthe cells treated with PNANFκB/MPOCs and those treatedwith PNANFκBrandom/MPOCs, the latter showed 70% less increase ofIl6 mRNA level upon LPS/rmIFN-γ stimulation (Figure 6a).
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