The use of additions economizes energy and enhances biogas production Clay minerals and surface-active materials can influence the microorganic and enzymatic transformation of the AD process [17], and some active minerals and materials used in enzymatic transformation of the AD process was given in Table 1[18] and [19]. Zeolite is widely used in mesophilic AD because it immobilizes microorganisms, maintains ammonia/ammonium ion equilibrium, and releases micronutrients [20], [21] and [22]. The effects of iron, nickel, cobalt, and other metals on AD have been studied [23]. Natural magnetite (M) and natural zeolite (Z) contain several types of metals that are essential for methanogens and M has not yet been explored in the practical biogas production.