which belongs to cationic surfactant type antimicrobial
agent, followed by another freeze-drying step.Somephysical and antimicrobial properties of the prepared
BC films were investigated and the results showed that the drug-loading capacity of the BC dry film
was about 0.116mg/cm2 when soaked in 0.102% benzalkonium chloride solution. High water absorbing
capacity, an important quality for wound dressings was also achieved with a swelling ratio of 26.2 in
deionized water and of 37.3 in saline solution. With respect to the antimicrobial effect, a stable and
prolonged antimicrobial activity for at least 24 h was obtained especially against Staphylococcus aureus
and Bacillus subtilis, which were general Gram-positive bacteria that found on the contaminated wound.