chlorogenic acid content was detected by the method of Kahle. Kraus. and Richling(2005) with some modifications. An Agilent 1100 series HPLc system was used. The mobile phase consisted of aqueous formic acid(0.1:99.9, vlv) (A) and methanol(B) The gradient applied was(10:90-90:10, vlv) Bin 40 min at a flow rate of 1 mblmin. column of Agilent Zorbax Eclipse XDB C18 was used and the injection volume of 20 HL was used Sample was filtered by using a syringe filter of 0.45 um diameter before injection into column. The peak was identified by com parison of retention time and UV spectra(320 nm) with that of standard. A suitable calibration curve was prepared using con centrations(10-80 uglmL) of standard solution and the results were expressed as ug of chlorogenic acid equivalent per mL of sample