Ascorbic acid was determined by the standard method as described in AOAC (1990). The standard dye solution for Ascorbic acid determination was prepared by weighing 50 mg of 2, 6 dichlorophenol indophenol dye and 42 mg of sodium bicarbonate, dissolving in hot distilled water and volume made up to 250 ml. Ascorbic acid standard solution was prepared by taking 50 mg of ascorbic acid in 50 ml volumetric flask and the volume was made up with 0.4% oxalic acid.