MIC was determined by incorporating various concentration of
the extract ranging from 0.5 to 5 mg/ml in 10 ml of SDA. Culture
medium and extract were mixed thoroughly and allowed to solidify.
A drop of olive oil was overlaid and 100 ml of the inoculum
was inoculated on each plate. The plates were incubated at 30 C for
5 days. Negative control was maintained with solvent. The concentrations
which completely inhibit the growth of organisms
considered as MIC.11