The total pectin contentwas determined with the K-PECID 11/11
kit (Megazyme International Ireland Ltd., Wicklow, Ireland). The
pectate is incubated with pectate lyase, which cleaves the polygalacturonic
acid, releasing unsaturated oligosaccharides, which
absorb strongly at 235 nm. From the increase in absorbance (DA)
the amount of unsaturated product produced can be calculated as: