Observational consequent and statistical analysis: The size, quality and degree (amount) of calluses were measured exactly by direct observation with binocular. The number of shoots developed from the callus was counted. All statistical analyses were performed using Microsoft Excel 2010. Standard errors were calculated and the mean comparison was determined by Duncan’s Multiple Range Test.
RESULTS
Callus formation from leaf, cotyledon and hypocotyl explants: Callus induction trend from aseptic in vitro grown tissues of sugar beet is considered one of the important aspects in genetic manipulation by gene transfer as well as production of hybrid plants. In this experiment, the early results were observed according to the effect of different growth regulator concentration on callus formation in three types of explants in two genotypes of sugar beet (Table 1).