In vitro culture of Curcuma mangga from rhizome bud
In vitro propagation of Curcuma longa (Turmeric)
Experiments were conducted for the standardization of in vitro culture technique for the mass propagation of Curcuma
longaLinn.(Syn. C. domesticaValet.) is one of the important spice crops. It is a rhizomatous perennial belonging to family
Zingiberaceae. Scooped rhizomatous buds were used as explants and they were cultured on MS medium supplemented
with different concentrations of BAP, Kn and IAA both in individual and in combined form for shoot inductions and the
best results were obtained from MS medium supplemented with BAP+IAA at the concentration of 2.0mg/l and 0.5mg/l
respectively. Best root formation of in vitro developed shoots could be achieved on halt strength MS medium
supplemented with IBA at concentration 1.0mg/l. The in vitro developed plantlets were transferred to pot and they were
grown in greenhouse for hardening and finally they were planted in the open field. Around 90% of plants were successfully
established in natural field condition.