2.3. Metal biosorption experiments
All biosorption experiments were performed by the
batch technique. Four different morphological forms
of Spirulina biomass (M—mixotrophic, L—lyophilized
cells from SIGMA, C—heterotrophic, A—photoautotrophic)
were used in biosorption studies.
Biosorption experiments were performed for Cr3+,
Cd2+ and Cu2+ ions at various initial concentrations at
35C (temperature of cellular growth) and sorbent concentration
1g l1. The experiments were performed in
250ml Erlenmeyer flasks containing 100ml of chromium(
III), cadmium(II) and copper(II) synthetic solutions
in thermostated water bath shaker at 150rpm.
The contact time was evaluated from kinetic experiments
as 30min. Samples of sorbent suspension (5 ml)
were taken to determine residual concentration of metal
ions in the solution. The biomass was separated from the
metal solution by centrifugation and the samples were
stored for further analysis. The samples were analyzed
for metal ions content.
Also the influence of pH on biosorption performance
was studied. These experiments were done for all
morphological Spirulina forms and for Cr3+ ions.
Washed algae were pelleted by centrifugation, and suspended
in solution containing ca 2meql1 of heavy metal
ions. The suspension was then titrated with 0.1M
NaOH. During titration, after pH was recorded, a sample
of 2.5ml was taken from the solution for metal
analysis
2.3. Metal biosorption experimentsAll biosorption experiments were performed by thebatch technique. Four different morphological formsof Spirulina biomass (M—mixotrophic, L—lyophilizedcells from SIGMA, C—heterotrophic, A—photoautotrophic)were used in biosorption studies.Biosorption experiments were performed for Cr3+,Cd2+ and Cu2+ ions at various initial concentrations at35C (temperature of cellular growth) and sorbent concentration1g l1. The experiments were performed in250ml Erlenmeyer flasks containing 100ml of chromium(III), cadmium(II) and copper(II) synthetic solutionsin thermostated water bath shaker at 150rpm.The contact time was evaluated from kinetic experimentsas 30min. Samples of sorbent suspension (5 ml)were taken to determine residual concentration of metalions in the solution. The biomass was separated from themetal solution by centrifugation and the samples werestored for further analysis. The samples were analyzedfor metal ions content.Also the influence of pH on biosorption performancewas studied. These experiments were done for allmorphological Spirulina forms and for Cr3+ ions.Washed algae were pelleted by centrifugation, and suspendedin solution containing ca 2meql1 of heavy metalions. The suspension was then titrated with 0.1MNaOH. During titration, after pH was recorded, a sampleof 2.5ml was taken from the solution for metalanalysis
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