The effects of different operating conditions on four extraction techniques were studied
and compared. The criteria analyzed were total phenol and flavonoid contents, individual
flavonoids and antioxidant activity. The common operating conditions of extraction are ratio
m/v: 5 g:50 ml; 80% ethanol with mechanical agitation. It appears that the highest values for
the total phenol and flavonoid contents were reached when ultrasound assisted extraction
(UAE) was carried out at 125 W during 30 min at 35
◦
C, microwave assisted extraction (MAE)
at 200 W during 180 s, supercritical CO2 extraction (SCE) at 80
◦
C, 10 MPa and high pressure
extraction at 50 MPa during 30 min at 35
◦
C. These conditions are not optimum to obtain the
highest antioxidant activity for MAE (300 W) and HPE (100 MPa).
© 2015 The Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.