Amino acids were analyzed by RP-HPLC after derivatization with diethyl ethoxymethylenemanolate, and determined according to the method described by Alaiz, Navarro, Giron, and Vioque (1992), using D, L a-aminobutyric acid as internal standard and a Novapack C18 column (300 3.9 mm i.d., 4 lm, Waters).Electro-spray-ionization high-resolution mass spectra were recorded with a micrOTOF-QII High Resolution-of-Flight mass spectrometer (UHR-TOF) with qQ-TOF geometry (Bruker Daltonic,Bremen, Germany).